FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004177196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6073042
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG148450.24444092433413106No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT132230.21773272768408322TruSeq Adapter, Index 7 (97% over 38bp)
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT97760.1609736932496103No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA81580.13433136144950092No Hit
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC63170.10401706426532206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)