FastQCFastQC Report
Mon 12 Sep 2022
EGAF00004177197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632568
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG128790.22865236602558547No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT112320.19941170705795297TruSeq Adapter, Index 7 (97% over 38bp)
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT87050.15454762374817313No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA71510.1269580766712448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)