FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004177198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5367383
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG122870.2289197547482637No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT108700.20251955189335286TruSeq Adapter, Index 7 (97% over 38bp)
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT81800.15240201789214594No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA68950.1284611141034653No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)