FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004177215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8654963
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG214110.24738407316126018No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT154290.17826765983863824No Hit
GTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCATAGCGTGGTCAA126720.14641310425012793No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT114420.13220160502130396TruSeq Adapter, Index 7 (97% over 38bp)
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC89590.10351286308214142No Hit
GTCAAAAAGCCTGGATAACGGAACGGTTTGGACGGCACCAGCCTCTTGAA89030.10286583547497545No Hit

[OK]Adapter Content

Adapter graph