FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004177230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12114244
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG503930.4159813852189208TruSeq Adapter, Index 7 (97% over 35bp)
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG255930.21126369916273768No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT129590.10697324570976116No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT123390.1018553035583566TruSeq Adapter, Index 7 (97% over 36bp)
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA122110.1007986961464537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)