FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004177235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10855122
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTATG379730.3498164276734983TruSeq Adapter, Index 7 (97% over 35bp)
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG271900.2504808329192431No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT153620.1415184463150207No Hit
CTTTGGGATATAGGTTTCTTCAAACTCCTGGTAGGTGTCAATGGCCAGCT124630.11481215964224077No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT124500.11469240050917898TruSeq Adapter, Index 7 (97% over 36bp)
CTCCTGGTAGGTGTCAATGGCCAGCTGGTGCGCGCGATGGGCTTGGAGCA121580.11200242613579102No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA116090.10694490582418142No Hit
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC110220.10153732035439121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)