FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004177253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12898822
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG399010.3093383256238438No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG354740.2750173620505811TruSeq Adapter, Index 6 (97% over 35bp)
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA189320.14677309292274907No Hit
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC156230.1211195875096191No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT130370.10107124511060002No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)