FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004177306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1913377
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG53740.28086467016170885TruSeq Adapter, Index 1 (97% over 35bp)
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG48590.25394890813467497No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA22670.11848161653453554No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT21310.11137376481477512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)