FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004177320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2936371
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG67810.23093130942922402No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA37270.1269253782985869No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT32350.110170002360056No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT30580.10414215369924304TruSeq Adapter, Index 1 (97% over 36bp)
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC29410.10015764356751922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)