FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004177322

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177322
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971233
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG69670.23448177911325027No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA38020.1279603450823278No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT33170.1116371553493112No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT30410.1023480824290791TruSeq Adapter, Index 1 (97% over 36bp)
GTCTGCTTGAGGATCTGCCCAGTCCGGCGGCTGCCGTCTTCCAGCCTCCC30180.10157399301905976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)