FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004177326

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177326
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5143499
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG216660.4212307613941405TruSeq Adapter, Index 1 (97% over 35bp)
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG125400.2438029053762818No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA64520.1254398999591523No Hit
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT57310.11142220500091474No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT55980.10883641661056025TruSeq Adapter, Index 1 (97% over 36bp)
CTTTGGGATATAGGTTTCTTCAAACTCCTGGTAGGTGTCAATGGCCAGCT54240.10545350548332953No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)