FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004177327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004177327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5113936
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG222060.4342252229984888TruSeq Adapter, Index 1 (97% over 35bp)
GTTGCGTTTCCTCCATGTTGGAGGGTGTCGGAATAGAGTCTGAGAAGCAG121910.2383878093116535No Hit
GTCGGAATAGAGTCTGAGAAGCAGAAGGAGGTCTGGGAGTCATGCAGGAA63870.12489401509913303No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT58470.1143346338319447TruSeq Adapter, Index 1 (97% over 36bp)
CTGGGAGTCATGCAGGAATGAATACTTCTGGTCCTTTGGGATATAGGTTT57740.11290716191989889No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)