FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004186656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004186656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64931506
Sequences flagged as poor quality0
Sequence length25-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG791790.12194234336717832No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG684860.1054742207889033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG257500.013.94214345
ATAGGGG276900.012.89144438
GGGAGGT402750.012.6627363
GGCATAG280200.012.584435
GGTCACT392450.012.2777597
GGAGGTC404400.012.2500534
AGGGATC281650.012.0953972
GTCACTC413600.011.7747068
GAGCTTA305100.011.69793544
TGGCATA311450.011.54177634
GTTGTCG43750.011.39774426
AGGTCAC456750.010.9180926
ACTCTCC459300.010.621613511
CAGGGAT364100.010.5093011
TCACTCT485200.010.2310369
GAGGTCA496200.010.2188375
CATAGGG363300.09.89423337
CACTCTC494300.09.7789910
GCATAGG368250.09.732236
GGGATCA361950.09.492683
Produced by FastQC (version 0.11.5)