FastQCFastQC Report
Sat 24 Dec 2022
EGAF00004186777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004186777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498506124
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCTGTTATTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATA24248440.4864221086278972No Hit
CCCGCTCTGACGCCCTAGACTCACCCGAGGGTAAGCCGCCATTTACATTC18865010.37843085755151123No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT15277310.30646183195133625No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC11755970.2358239835785849No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG11086490.22239425889179248No Hit
CCCCGGAACCCAAAGACTTTAATTTCTTTTAGGCCGCCGGCTGAGTCTAT10492550.21047986162753737No Hit
CCCTTGTCCGTTACAGTTCTTTATTAGTAATTAATCACGACACCTGACTG10461540.20985780307084054No Hit
CCCGCTGTTATTGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTAGATAG9991240.20042361605972966No Hit
CCCCACTTCAATCTTCAAGTATCTCGCTTGATTATTTGCTACTACCACCA9885140.19829525705084416No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG8663070.1737806133751729No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA8591870.17235234606666539No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA8421460.16893393269527818No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT8389100.16828479322753517No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT8387780.16825831411451225No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT7990330.1602854933031876No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT7910860.1586913303396048No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG7853590.15754249791322522No Hit
GCCCTCGACACTCAACACCTCGTACTGAATAGTACTCGATACTGCGTACA7769260.15585084366987637No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG7727120.15500551804655463No Hit
CCCCTTGTCCGTTACAGTTCTTTATTAGTAATTAATCACGACACCTGACT7416720.1487789144993533No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG7332310.14708565546127575No Hit
CTGCGGTCTAAGCGATAACCTTCCCCTTTACCGGTTTCACGTACTTTTAA7078820.142000662764175No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT7022470.140870285477175No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC6945950.13933529931921157No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA6846790.13734615625303734No Hit
CTCGCTTGATTATTTGCTACTACCACCAAGATCTGCACCAGAGGCCGCTC6844530.13730082080195266No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA6643780.13327378902972112No Hit
GCCGCAGGCTCCACTCCTGGTGTGCCTTTCCGTCAATTCCTTTAAGTTTC6247580.12532604313603177No Hit
CCGCTCTGACGCCCTAGACTCACCCGAGGGTAAGCCGCCATTTACATTCA6139040.12314873788792213No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG6114810.12266268568447918No Hit
CCCCGCATCGCCAACTCTGCTTACCAGAAAAGGCCCACTTGCCTAAAAAC6077550.12191525253960572No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC5959390.11954497072537468No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC5892470.11820255993485046No Hit
CCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCG5680230.1139450395197151No Hit
CCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCACGGCGACTACCA5606900.11247404455155702No Hit
GGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGC5485810.11004498713038878No Hit
CCTCGACACTCAACACCTCGTACTGAATAGTACTCGATACTGCGTACATG5458060.10948832395888482No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT5415650.10863758215335385No Hit
CCCGTGGTCACCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGG5406860.10846125533254232No Hit
ATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGG5388940.10810178131332245No Hit
CCCGTGACGACTTACTTTAAGTGATTACCCGACCCTTTTATAGGCGAGGG5255690.10542879509339788No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG5010860.1005175214256746No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)