FastQCFastQC Report
Fri 16 Dec 2022
EGAF00004186778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004186778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498506124
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT50893101.0209122325646696No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG18208040.3652520826404131No Hit
CTTACCAAGTCCGGACATGGTAATGATTGACAGACTAATAGCTCTTTCTT13774800.27632158035434684No Hit
CAAATGTTTACGTGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG12159340.24391555919983043No Hit
CCTAAATAACCCCCTGAACTTAAGCATATCAATATGGGGAGGAAAAGAAA11980140.24032081900763172No Hit
TCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA11102600.2227174244302764No Hit
CTCTCGACTAGTGTACTGATGAAGAACGTAGCAAACTACGTAAAGCCAGG10092510.20245508558687236No Hit
CGATGGTTTTAACAAATGTTTACGTGATGTGATTTCTGCCCAGTGCTCTG9736060.195304722073986No Hit
GTCCTAAATAACCCCCTGAACTTAAGCATATCAATATGGGGAGGAAAAGA9646770.19351357055746018No Hit
GGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAA9514210.19085442569207034No Hit
GATGGTTTTAACAAATGTTTACGTGATGTGATTTCTGCCCAGTGCTCTGA7985910.1601968283944291No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAG7725640.15497582934407442No Hit
CGTAATTTGAGAGTATTACGCAAAGCGGGAGGTAAGTACCTTCTAATGCT7368300.1478076124898317No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG6706840.13453876847458748No Hit
AGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAA6587600.13214682193151955No Hit
GGTGGATCTCTCGACTAGTGTACTGATGAAGAACGTAGCAAACTACGTAA6570030.13179436888923757No Hit
GTTTTAACAAATGTTTACGTGATGTGATTTCTGCCCAGTGCTCTGAATGT6379200.12796633166335986No Hit
CCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACTC6120480.12277642551087296No Hit
CTCGACTAGTGTACTGATGAAGAACGTAGCAAACTACGTAAAGCCAGGTG6038000.12112188214562436No Hit
GTTTGATCCTGGCTCAGGACGAACGCTGGCGGCGTGCTTAACACATGCAA6020470.1207702315007067No Hit
AGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCG5919080.11873635478147106No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC5812240.11659315142134542No Hit
CGTAGGTGTCCTAAGGGGAGCTCATTGAGAACAGAAATCTCGAGTAGAGT5785600.1160587547767016No Hit
GTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCA5715620.11465496058780614No Hit
GCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCGGCGGCTTTGGTGACT5655090.11344073277623366No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG5326710.10685345161376594No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5213900.10459049044701406No Hit
AGGACATTCAAGTTTCTTCCCTATCAACTTAAACCGAAGTCTATTGAACT5128450.10287636907746393No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC5093400.1021732683869697No Hit
CTTTAAATGGGTAAGAAGCCCGGCTCGCTGGCGTGGAGCCGGGCGTGGAA5003610.10037208690338978No Hit
CAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTC4999460.10028883817684052No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)