FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004186855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004186855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35128906
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT1772210.5044876717766275No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1190010.3387552120182735No Hit
GGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCC1151560.3278098099610617No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG957050.2724394548466724No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG953820.271519984140696No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG920440.2620178379594286No Hit
CAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGG888040.2527946643143399No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG843880.2402238202351078No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT815030.2320112103690334No Hit
GGCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAA796940.22686160508385889No Hit
CCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAATTGCAATCCCCG794320.22611578054836093No Hit
CTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTT776810.2211312814580676No Hit
CTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCG745540.21222978022714398No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA710030.20212129577846802No Hit
GCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAA681670.19404817218048295No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA661950.1884345615545215No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG624630.17781083191147484No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA606790.17273239308961116No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC602810.1715994229937021No Hit
GCCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAG592350.1686218181687753No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC568290.16177275773973718No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA565730.16104401315543387No Hit
GTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGC560810.15964345715747597No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC554830.15794115535507994No Hit
CCTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGG554340.15780166908699064No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC553240.15748853664842283No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT551020.1568565784542223No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA541930.15426896584823904No Hit
CGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTC541060.15402130655591723No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCG518960.14773019119923633No Hit
CCCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGT516310.14697582668814108No Hit
GTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGT511220.1455268774951318No Hit
CCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACT504970.14374771591236002No Hit
CTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAA486300.13843300443230427No Hit
CTCCCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACT486110.138378917920188No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA485330.1381568785546581No Hit
GTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCT484830.13801454562803633No Hit
ATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGG465390.13248064144098312No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC461270.13130781812561995No Hit
ATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGTGCGTCGGGTCTGCGA458910.13063600671196535No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT449270.1278918278866982No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT448940.12779788815512785No Hit
CGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAG445290.12675885779078916No Hit
CTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATT426960.12154093270083617No Hit
CCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGA424780.12092036114076539No Hit
CGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCC414070.11787158985252771No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT404640.11518719085644169No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA402080.11445844627213839No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA399570.11374393498049726No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC398400.11341087593220239No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT393150.11191638020267411No Hit
TACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGC390800.11124741544755194No Hit
CCGGAAGCTGCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTC387310.11025393161973221No Hit
CCAACTACGAGCTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGC383340.10912380818235558No Hit
GCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAG383020.10903271510931767No Hit
GTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAA382890.108995708548396No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC381040.10846907671989557No Hit
CTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCA380500.1083153571591441No Hit
GGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCC378550.1077602587453193No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG376000.10703436081954842No Hit
CGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCA370030.10533490567568486No Hit
CGGGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACC368500.10489936692022234No Hit
GTTCGATTAGTCTTTCGCCCCTATACCCAGGTCGGACGACCGATTTGCAC363300.10341910448335626No Hit
GCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAA363280.10341341116629137No Hit
CGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCC361240.10283269282567468No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT361160.1028099195574152No Hit
CACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAACTG359380.10230321433864181No Hit
ACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAG358930.10217511470468224No Hit
GGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGG357500.10176804253454405No Hit
CTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTT354080.10079448531645137No Hit
GGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGTGGCTCGCCTCGCGGCGG353890.1007403988043351No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGA352950.10047281290228624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)