FastQCFastQC Report
Tue 19 Sep 2023
EGAF00004188060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004188060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42144852
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1877650.44552297870212Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1569350.3723705092142689Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGCAGAAGACGGCATACGAGATTCTGCTTGAGATCGGAAGAGCGTCGT1040760.24694831055522512RNA PCR Primer, Index 40 (96% over 28bp)

[OK]Adapter Content

Adapter graph