FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004196421

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004196421
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35886049
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT1813150.5052520549141534TruSeq Adapter, Index 19 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA604110.16834118462024059No Hit
GCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCCCTCGCGCG603080.16805416500434472No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)