FastQCFastQC Report
Wed 14 Sep 2022
EGAF00004196433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004196433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31093337
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT1150960.3701629066060037TruSeq Adapter, Index 3 (97% over 36bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA356390.11461941186949474No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA336270.10814857215229103No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG313940.10096696922559324No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)