FastQCFastQC Report
Sat 1 Apr 2023
EGAF00004196519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004196519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32480195
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT1486310.45760501129996295TruSeq Adapter, Index 19 (97% over 38bp)
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA445230.13707737899972583No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA373600.11502393997326679No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG327720.10089840901509366No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)