FastQCFastQC Report
Wed 27 Sep 2023
EGAF00004196602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004196602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33979933
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT2423920.7133386637342692No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT1503040.4423316549800142No Hit
CCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT902360.2655567331460012No Hit
GCGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT700800.20623937074861215No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT528810.15562420326137782No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA492650.14498262842366405No Hit
GAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTCGGTG432200.12719271694855902Illumina Single End PCR Primer 1 (96% over 27bp)
GGGGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCCCTCGCTGCGATCTA385180.11335513816345665No Hit
GCCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT384290.11309321887126733No Hit
GGGGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG353980.10417324836985405No Hit
GGGGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAAT350380.10311379954751529No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)