FastQCFastQC Report
Tue 26 Sep 2023
EGAF00004196621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004196621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33080563
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG817360.24708164731053703No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG645020.19498458959117473No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG625830.18918360004937038No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT471530.14253989570854642No Hit
GGGGTGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGGG423980.12816589608828605No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG423880.1281356668566977No Hit
TGGGGTGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG397410.12013398925526146No Hit
CCTTGGTCCGTGTTACAAGACGGGTCGGGTGGGTAGCCGACGTCGCCGCC392820.11874646752535621No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA376520.11381910277645517No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT361800.10936935988665006No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG341990.10338094910899794No Hit
GTGGGGTGGGGGTGGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGG335790.10150673675052024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)