FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004198247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273563996
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAAT11608600.06.4148991
GATCGGA7021750.06.3173695140-144
ATCGGAA6822300.06.246695140-144
AGATCGG7435500.06.242743140-144
TCGGAAG6586200.06.225563140-144
ACACGTC4061400.06.2060494140-144
CGTCTGA3528900.06.184937140-144
CGGAAGA6422800.06.1272697140-144
ACGTCTG3873950.05.984156140-144
GTCACGC1845500.05.919161140-144
TAGATCG1876900.05.844682140-144
CACGTCT4174400.05.811445140-144
GCACACG4967050.05.70844140-144
CAGATCG2279050.05.664967140-144
ACGCTTC1642500.05.656664140-144
AGTCACG2092550.05.592577140-144
CACACGT4860950.05.5848236140-144
CTAAAAT6155150.05.3374291
CACGCTT1863250.05.3203096140-144
TATGCCG720350.05.3147016140-144
Produced by FastQC (version 0.11.5)