FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004198252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329975060
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCGT2167600.06.6048636140-144
TGCGCCG2305650.06.591007140-144
CGCCGTG2142050.06.432891140-144
CGTCGTG6119700.06.4173555140-144
AGCGTCG6860900.06.408938140-144
GCGTCGT6377200.06.360106140-144
CAAAAAT13930400.06.3354911
GTCGTGT5884150.06.2655525140-144
GAGCGTC7449600.06.2212305140-144
TCGTGTA5615600.06.1736126140-144
CGTGTAG5421750.06.171771140-144
AGAGCGT7925400.06.0041814140-144
GATCGGA10801800.05.986829140-144
TGTGCGC2904250.05.958422140-144
GTGCGCC2745100.05.930368140-144
TCGGAAG10111200.05.925039140-144
ATCGGAA10444600.05.918312140-144
AGATCGG11377150.05.899495140-144
AAGAGCG8600400.05.8775296140-144
CGGAAGA9790950.05.841011140-144
Produced by FastQC (version 0.11.5)