FastQCFastQC Report
Thu 23 Mar 2023
EGAF00004198428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50936569
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGATCTCGAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGG2998500.5886733360466426No Hit
ATAGATCTCGCTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTG1018410.1999369058406741No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN955150.1875175377438555No Hit
ATAGATCTCGGGCTCCTCGGCTTTGACAGAGTGCAAGACGATGACTTGCA877130.1722004479728503No Hit
ATAGATCTCGCTCGGCTTTGACAGAGTGCAAGACGATGACTTGCAAAATG868200.1704472870954461No Hit
ATAGATCTCGCTCCTCGGCTTTGACAGAGTGCAAGACGATGACTTGCAAA793360.15575450321359494No Hit
ATAGATCTCGCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCT624650.12263291624530108No Hit
ATAGATCTCGCCTCGGCTTTGACAGAGTGCAAGACGATGACTTGCAAAAT572020.11230045745719544No Hit
ATAGATCTCGCCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC550130.10800295559757862No Hit
ATAGATCTCGCAGCATTCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCT542340.10647360249175794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)