FastQCFastQC Report
Tue 4 Apr 2023
EGAF00004198488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47267566
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACAGATCTCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGC6042121.2782803328608034No Hit
ACAGATCTCGCACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGG2638250.5581522856497413No Hit
ACAGATCTCGGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCAT2346020.4963276509731853No Hit
ACAGATCTCGACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCA2009900.4252175794285663No Hit
ACAGATCTCGGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCT2002290.42360759595702474No Hit
ACAGATCTCGCAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTG1386800.293393571397351No Hit
ACAGATCTCGCTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCAC1326410.2806173687894147No Hit
ACAGATCTCGCAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCG1206260.25519824735633734No Hit
ACAGATCTCGAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGG1124860.2379771363729624No Hit
ACAGATCTCGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA1102530.23325296673833384No Hit
ACAGATCTCGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATG945320.19999337389194102No Hit
ACAGATCTCGGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGA926420.19599486040808617No Hit
ACAGATCTCGACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCC915020.19358305862417372No Hit
ACAGATCTCGCTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAA883230.18685751663201783No Hit
ACAGATCTCGCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTG872460.18457899863090052No Hit
ACAGATCTCGGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG778880.16478106784681912No Hit
ACAGATCTCGTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACC741550.15688347481230575No Hit
ACAGATCTCGCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAA683800.1446657947227492No Hit
ACAGATCTCGAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGC634650.13426754404912664No Hit
ACAGATCTCGGGAACGTCTGAGGTTATCAATAAGCTCCTAGTCCAGACGC628290.13292201252757546No Hit
ACAGATCTCGGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC612730.12963011465409494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)