FastQCFastQC Report
Thu 9 Feb 2023
EGAF00004198559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83658111
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9903451.1838003370647467No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA1428130.17071028534220667No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1332010.1592206642103119No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG1290340.15423967677204664No Hit
ACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCT956830.11437384714555651No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)