FastQCFastQC Report
Fri 24 Mar 2023
EGAF00004198568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45490382
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC923510.203012144413296No Hit
ATCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGA922480.20278572292490313No Hit
ATCACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTG658650.14478884789316562No Hit
ATGTTTGCCGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAA602800.1325115273817661No Hit
ATCAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC595000.13079687921723762No Hit
ATGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACC571950.12572987406436817No Hit
ATCTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCC568340.12493629972155432No Hit
ATGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTG542240.11919882317101668No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN527360.11592780205714694No Hit
ATGCCAGAACACAGGTGTCGTGAAAACTACCCCTAAAAGCCAAAATGGGA486910.10703581253725238No Hit
ATCCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTT482510.10606857511110811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)