FastQCFastQC Report
Thu 5 Jan 2023
EGAF00004198580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41701777
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1959410.46986247132826015No Hit
ATAGATCTCGGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTC1140920.2735902597148318No Hit
ATAGATCTCGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGT778720.18673544774842568No Hit
ATAGATCTCGGGTGAGTCGACCCCTAAGGCGAGGCCGAAAGGCGTAGTCG723540.17350339770892736No Hit
ATAGATCTCGCATGGCTCAGATTGAACGCTGGCGGCAGGCCTAACACATG678190.16262856136802034No Hit
ATAGATCTCGAAAGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACG631700.15148035538149848No Hit
ATAGATCTCGGCCCGCTCGCCGGAAGACCAAGGGTTCCTGTCCAACGTTA613490.1471136349897032No Hit
ATAGATCTCGGTCCAACGTTAATCGGGGCAGGGTGAGTCGACCCCTAAGG581320.1393993354287996No Hit
ATAGATCTCGGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCCTA550620.13203753883197833No Hit
ATAGATCTCGGTCAGATGTGAAATCCCCGGGCTCAACCTGGGAACTGCAT550220.13194161965807838No Hit
ATAGATCTCGAGCGGGTGAAAAGCCCGCTCGCCGGAAGACCAAGGGTTCC421300.10102686991012397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)