FastQCFastQC Report
Fri 15 Sep 2023
EGAF00004198601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43896922
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCACCACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGG883460.2012578467346754No Hit
GTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTTTCTTGCCAT828690.188780889922077No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN817240.18617250658257997No Hit
CAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCACA738070.1681370734831932No Hit
CTTTAATAGAAATTGGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGG694860.1582935587146634No Hit
GTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCC674840.15373287448263456No Hit
CTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACT635540.1447800827584221No Hit
GTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGTCCA630750.1436888900775321No Hit
GGACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCC624790.14233116390256245No Hit
GCCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACA544170.12396541151564112No Hit
CTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGA540600.12315214264909052No Hit
CTTGCCATGAGCCTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGG530470.12084446376445254No Hit
CACCAACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGAC523880.11934321955420929No Hit
GCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAGG506620.11541128100052207No Hit
CTTGAGGTTGTCCAGGTGAGCCAGGCCATCACTAAAGGCACCGAGCACTT506180.11531104618223574No Hit
CCTGCACTGGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAG502360.11444082571438609No Hit
GACAGCAAGAAAGCGAGCTTAGTGATACTTGTGGGCCAGGGCATTAGCCA493820.11249535901401014No Hit
CAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCTCTGGGTCCAAGGG487690.11109890575015716No Hit
GCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACC483330.11010566982350152No Hit
CCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGACTCAAAGAACCT477190.10870693849559657No Hit
CTTCACCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAA472620.10766586322384973No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)