FastQCFastQC Report
Mon 27 Mar 2023
EGAF00004198620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50641359
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGA9821441.9394108282125684No Hit
TACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTG6873341.3572582047018131No Hit
TAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTC5909241.1668802174128068No Hit
TACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTT5205151.0278456389766317No Hit
TAGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTG5194391.0257208934697033No Hit
TACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGAC4910710.9697034394357387No Hit
TACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCC4680890.9243215609596891No Hit
TACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACG3846970.7596498348316443No Hit
TAGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG3456400.6825251273371238No Hit
TACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACC3312690.6541471369281382No Hit
TATGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCT2944030.581348932598748No Hit
TAGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACC2718560.536826035809979No Hit
TACCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA2564940.50649114689043No Hit
TAAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAG2393290.4725959269773941No Hit
TACTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCG2267280.4477131034338948No Hit
TACCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAG1467310.28974538380772913No Hit
TAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTC1463490.2889910596593587No Hit
TAAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACT1460660.28843222789498996No Hit
TAACACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCT1354150.26740001191516205No Hit
TAAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGT1215300.23998171139127605No Hit
TAACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGAC1206670.2382775707105333No Hit
TACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCC1156430.22835682589007927No Hit
TACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAA1128120.22276653357584658No Hit
TACTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTG1122300.22161727531838155No Hit
TACTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTC1072630.21180908671901952No Hit
TAGTCAACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCC986790.19485851475668337No Hit
TACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGT924210.1825010264831163No Hit
TATGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGA869600.17171735063429083No Hit
TAGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGG859520.16972688272445452No Hit
TAGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCT850910.1680266913848027No Hit
TACAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTC849120.1676732253571631No Hit
TATAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAG837940.16546554368732483No Hit
TAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGG790510.15609968129014865No Hit
TACAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTT748200.14774485021225436No Hit
TACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTG719660.1421091404754758No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN710670.14033391165509598No Hit
TAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAA667520.13181320825138204No Hit
TAGGAGAAGTCTGCCGTTACTGCCCTGTGGGGCAAGGTGAACGTGGATGA656970.12972993082590853No Hit
TACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCC628290.1240665757014933No Hit
TACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGT623340.12308911378148442No Hit
TACTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACC616420.12172264176401743No Hit
TACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTA615830.12160613620183455No Hit
TAGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGT611050.12066224368109868No Hit
TACTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG604110.11929182232254076No Hit
TACTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCC582170.11495939514577402No Hit
TACTTCAAGCTCCTAAGCCACTGCCTGCTGGTGACCCTGGCCGCCCACCT562640.11110286357046618No Hit
TAGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGCTG556650.10992003591372813No Hit
TACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCT546920.10799868147298337No Hit
TACACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCAAGGCCGCCTG532880.10522624402713994No Hit
TACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTG508220.1003567064620047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)