FastQCFastQC Report
Fri 24 Mar 2023
EGAF00004198622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55874997
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTT9828091.758942376319054No Hit
ATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTA6002161.0742121382127323No Hit
ATCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTC2176510.3895320119659246No Hit
ATCTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATG2034140.3640519211124074No Hit
ATGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCT1409570.25227204933899144No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1388960.24858345853691946No Hit
ATGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC1326040.23732260782045322No Hit
ATCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA1075800.1925369230892308No Hit
ATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAA869640.15564027681290077No Hit
ATCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTG754730.1350747276102762No Hit
ATCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATG685150.122621930521088No Hit
ATCGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGA657430.11766085642921824No Hit
ATCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAA647210.11583177355696324No Hit
ATAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCAT645560.11553647152768529No Hit
ATCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTA630840.11290201948467218No Hit
ATGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGC611740.10948367478212123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)