FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004198656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5988677
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGATCTCGAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGG1051421.7556799273028085No Hit
GTAGATCTCGGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGT321650.5370969247464841No Hit
GTAGATCTCGCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCT247820.41381426982954667No Hit
GTAGATCTCGGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCT178040.29729437737249814No Hit
GTAGATCTCGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTC154670.25827073325210226No Hit
GTAGATCTCGCTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAGAGG138320.23096921072884713No Hit
GTAGATCTCGCCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCC134740.2249912626778836No Hit
GTAGATCTCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGC101070.16876849427678267No Hit
GTAGATCTCGATAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCT91090.15210371172130338No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN83240.13899564127435826No Hit
GTAGATCTCGTAGAAGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTG82160.13719223795172122No Hit
GTAGATCTCGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCC79330.13246665331925564No Hit
GTAGATCTCGGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAGCTAG75250.12565379632262685No Hit
GTAGATCTCGGACCTGGGACAGAGGACTGCTGTCTGCCCTCTCTGGTCAC68090.11369790022069982No Hit
GTAGATCTCGCAGAGGACTGCTGTCTGCCCTCTCTGGTCACCCTGCCTAG67620.11291308581177445No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)