FastQCFastQC Report
Tue 14 Feb 2023
EGAF00004198660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004198660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87357385
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGATCTCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGC3398040.3889814238372634No Hit
ATAGATCTCGCAACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTG3110700.35608895573053156No Hit
ATAGATCTCGACTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCA2577560.29505919848676787No Hit
ATAGATCTCGCACAACTGTGTTCACTAGCAACCTCAAACAGACACCATGG2544200.29124040285775493No Hit
ATAGATCTCGCTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCAC2498250.28598040108457917No Hit
ATAGATCTCGGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCT1637080.18740029821176538No Hit
ATAGATCTCGCCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT1340330.15343064584637006No Hit
ATAGATCTCGGACACAACTGTGTTCACTAGCAACCTCAAACAGACACCAT1280920.14662984703582874No Hit
ATAGATCTCGCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATG1264990.14480630343960044No Hit
ATAGATCTCGTGTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACC1179190.13498458086857798No Hit
ATAGATCTCGCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAA991610.11351186851575285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)