FastQCFastQC Report
Thu 5 Jan 2023
EGAF00004455688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20155367
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAAC9721174.823117336439471No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG3662351.8170594462507184No Hit
TACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGATCTGATGTGGAGG1779450.8828665833770231No Hit
GATATTCGCAAGAACACCAGTGGCGAAGGCGGCTCACTGGCCCGATACTG1199050.5949035807683383No Hit
ACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGATCTGATGTGGAGGC1163390.5772110227514091No Hit
AGCTTAGGGTGTGATCCGCAAGGGTCACGCGGTAGCGGAGCGTTCTGTAA1139170.5651943722979591No Hit
CTTGACATCCCGGTCGCGGACTCCAGAGACGGAGTTCTTCAGTTCGGCTG1127280.5592951991397626No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA934460.4636283725322392No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA810020.4018879934064213No Hit
AAGCTTAGGGTGTGATCCGCAAGGGTCACGCGGTAGCGGAGCGTTCTGTA702400.3484927860653691No Hit
TGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGG643550.31929460773400953No Hit
ATGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAA638430.31675434141189296No Hit
TCTCCAACATAGACTCAGTGAAATTGAATTCCCCGTGAAGATGCGGGGTT616980.3061120147303693No Hit
CGCAGAACCTTACCAGCCCTTGACATCCCGGTCGCGGACTCCAGAGACGG434460.21555548951304138No Hit
AACTTGAGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAA427660.21218169830398026No Hit
CCTTGACATCCCGGTCGCGGACTCCAGAGACGGAGTTCTTCAGTTCGGCT309390.1535025385546192No Hit
ATAGACTCAGTGAAATTGAATTCCCCGTGAAGATGCGGGGTTCCTGCGGT305200.15142368779491833No Hit
CTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCG278270.13806248231550436No Hit
GTAATGACTTCCCCGCTGTCTCCAACATAGACTCAGTGAAATTGAATTCC249960.12401659567895737No Hit
CTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCA245120.12161525017133153No Hit
TCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC218330.10832350509916291No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA210260.10431960876723306No Hit
AATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCGC203280.10085651132028506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)