FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004455689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20155367
Sequences flagged as poor quality0
Sequence length50-120
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAAC7287493.6156573085471475No Hit
TCAAAGGCAGTTCTGGAGTTGAGCTCCAGGATTTCACCCCTGACTTAAAG3705921.838676517277011No Hit
TACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGATCTGATGTGGAGG1831780.9088298913138124No Hit
ACTCTACCAGTCCACCTGTGTCGGTTTCGGGTACGATCTGATGTGGAGGC1225510.6080315977377142No Hit
CTTGACATCCCGGTCGCGGACTCCAGAGACGGAGTTCTTCAGTTCGGCTG1123990.5576628795694963No Hit
AGCTTAGGGTGTGATCCGCAAGGGTCACGCGGTAGCGGAGCGTTCTGTAA963930.47824978825739073No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA930630.4617281342483121No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA849820.4216345948947493No Hit
GATATTCGCAAGAACACCAGTGGCGAAGGCGGCTCACTGGCCCGATACTG752820.373508455589025No Hit
AAGCTTAGGGTGTGATCCGCAAGGGTCACGCGGTAGCGGAGCGTTCTGTA603230.2992900104473414No Hit
TGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGG593100.2942640538373724No Hit
ATGCAAGTCGAGCGGGCATAGCAATATGTCAGCGGCAGACGGGTGAGTAA491300.2437564148546638No Hit
TCTCCAACATAGACTCAGTGAAATTGAATTCCCCGTGAAGATGCGGGGTT423400.2100681173406567No Hit
CGCAGAACCTTACCAGCCCTTGACATCCCGGTCGCGGACTCCAGAGACGG371760.18444714998243394No Hit
CCTTGACATCCCGGTCGCGGACTCCAGAGACGGAGTTCTTCAGTTCGGCT335520.1664668274212025No Hit
AACTTGAGAGTTTGATCCTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAA319540.15853841807990895No Hit
CTGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCG275230.13655419918674763No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCG272110.13500622439670784No Hit
AATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCGC268800.13336398191112075No Hit
TTAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGC241680.11990850873615946No Hit
AGAGCTTTACAACCCTAGGGCCTTCATCACTCACGCGGCATGGCTGGATC213620.10598665854112208No Hit
AAGAGCTTTACAACCCTAGGGCCTTCATCACTCACGCGGCATGGCTGGAT212600.1054805898597629No Hit
ATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAAAGCGTTAGCTGCGCC210550.1044634910393842No Hit
CTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCA209590.10398719110398735No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)