FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004455693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23912564
Sequences flagged as poor quality0
Sequence length50-120
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTTTGGGAGGCAACCGCCCCAGTTAAACTACCCACCAGGCACTGTCC1547130.6469946091937276No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA1463200.6118959054328093No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA1038520.4342988899057416No Hit
ATAACCAGAAGTACAGGAATATTAACCTGTTTTCCATCGACTACGCTTTT866280.362269809293558No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA764550.31972731991433456No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCC632500.26450530357179597No Hit
TCATAAACAAGTATCGGAATATTAACCGATTTCCCATCGACTACGCCTTT612170.25600349673920375No Hit
TGTGGATCGGAGTGAAAGGCTAATCAAGCTCGGAGATAGCTGGTTCTCCT509790.21318918372785117No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA509250.2129633610180824No Hit
ACACGACGCTCCCCTACCCATCCACACACCTGCCAGAAAATCCTTGGATC473160.19787087658186714No Hit
GAGAAGGTACGCTGCCGATGGTGAGTAGTCACGCACTACAAAGCTATTGG420110.17568588629809836No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC389020.162684352878261No Hit
TCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTCAAATGAATTGAC387540.16206543137741314No Hit
CCCACGAATGCAACACCTGCCGGCTATCACACACCCATGGTTTAGCCTCT385650.16127505189322233No Hit
AGAAGGTACGCTGCCGATGGTGAGTAGTCACGCACTACAAAGCTATTGGC379700.15878682018373272No Hit
CGATTGGAATTTCTCCGCTACCCACAGTTCATCCGCTCACTTTTCAACGT371220.1552405672599559No Hit
CGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGA362430.15156467537316365No Hit
TGTGGATAGGGGTGAAAGGCTAATCAAACTCGGTGATAGCTGGTTCTCCC359930.15051919986497475No Hit
GTTAGGTTTAGTAGGCAAATCCGCTAGACTATTAATGAGATCTGATAGCA358170.14978318510720975No Hit
TGCGGGAATGACAGCTCGCCCACTCGGTGAAGCAGATCCCCGTGATCGAT337370.14108482887907797No Hit
TCAAAGGCAATTCCGAGGTTGAGCCCCGGGATTTCACCCCTGACTTAAAT306150.12802893073281477No Hit
GGAGGTAGAGCACTGTTTGGACGAGGGGCCCCTCTCGGGTTACCGAATTC300850.12581252265545426No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC296080.1238177553858298No Hit
AAAAGTTAGCGGATGACCTGTGGATCGGAGTGAAAGGCTAATCAAGCTCG292440.12229554304590674No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA279750.11698870936633982No Hit
AATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCG277880.1162066936862145No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT273810.11450465955888293No Hit
TAGCGAAAGACGAGTGAGAAACTCGTCCGCCGAATGACCAAGGGTTCCTG273120.11421610831862279No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266740.11154805482172468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)