FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004455694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8551894
Sequences flagged as poor quality0
Sequence length50-120
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGC2006372.346111867148961No Hit
ACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTG1605121.8769175576778665No Hit
AGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGA769760.9001047019525733No Hit
TAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAG601480.7033295782197487No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT517990.6057020819013893No Hit
CCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGAT515960.6033283387282395No Hit
CTCCGAGAGCCACCCCAATGCCACTTTCAGCGCCGTGGGTGAGGCCAGCA513750.6007441158648599No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACC337030.394099833323472No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTG302530.3537578926960507No Hit
TGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGG286250.3347211740463575No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG252770.29557195166357303No Hit
CAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGC239180.2796807350512062No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG234970.27475784896304845No Hit
GGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGAT230250.26923860375257225No Hit
CGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGG224470.2624798670329637No Hit
ACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCG220720.25809487348650484No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA215230.2516752429344891No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC199490.23326996335548594No Hit
GGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGACAGCCTGCGAA187740.21953031690991492No Hit
CTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGC173100.20241130210453964No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACC171470.20050529157634553No Hit
TCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGCTACTCT171220.2002129586732483No Hit
CTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTT164100.19188731759303845No Hit
ACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAAC162970.19056597287103885No Hit
GCGGGATGAACCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCA161150.18843778933649083No Hit
ATGCAGTCAGCAATGACTTAAATTAGTCAGGATAGCAGGCATCTGGGGTT157130.18373707625468697No Hit
CCTCTACCACCCCTGGGGGCCTCCTCCAAGGAGCAGGTGGTTAGATGTCC138270.16168348204503002No Hit
ACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTTGCAGGGACAGCAGA135770.1587601530140575No Hit
GGGTCAAGAAGCGGGTGGACCACTGTCCCATCAAATCGCGCAAAGGGGAT134700.15750896818880122No Hit
TAGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAG129810.15179093660421888No Hit
CGGGGGCCCTGGCCCTGACCCAGACCTGGGCAGGCTCCCACTCCATGAGG123540.14445922739453973No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA121930.1425766034985934No Hit
TCGGGAATTCACATACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTG118340.13837870301011684No Hit
GCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAACACCCA116120.13578278683061318No Hit
TGGACAGGCGATCATCATCACCTGCACTGGAACCAGCAGTGACGTTGGTG114870.1343211223151269No Hit
GCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAG112380.13140948660027826No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAG111810.1307429675812165No Hit
GCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATG107640.1258668547575543No Hit
CCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGG103830.12141170131435212No Hit
AGACACTGGCGTGCCTGCTTCAAAGCCTCCCACCTATCCTACACAGGTAA100800.11786862652881339No Hit
TCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTC98540.1152259370848142No Hit
AGCACGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTACTGCTCTG96950.11336669982111565No Hit
TAGTGATCAGTACCATATACAGTTTCTCCATAGGCTGGTAGACATATCTT96900.1133082332404962No Hit
TGCCAGTGAGTGTCTTCACGAAGATTTGCATCCCACCTCTGAGACGGAGC96640.11300420702127505No Hit
TGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGC94640.11066554379649701No Hit
GACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGAT91550.10705230911421493No Hit
ACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGAGGACTCAC88830.10387172712851679No Hit
TGAGGAGCAGCCTTGTCTCCGGAAGGACTCTCTCAGAATGTTCCTCAAAT88100.1030181150514728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)