FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004455695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8551894
Sequences flagged as poor quality0
Sequence length50-120
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAGACCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGC1432661.6752546278052556No Hit
ACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTG1308471.5300353348626632No Hit
CCAGCGAGCCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGAT506150.5918571956107033No Hit
TAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAG497060.5812279712540871No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT491300.5744926211667264No Hit
CTCCGAGAGCCACCCCAATGCCACTTTCAGCGCCGTGGGTGAGGCCAGCA448900.5249129608014319No Hit
AGCCCTGGACTCCAAGGCCTTTCCACTTGGTGATCAGCACTGAGCACAGA342330.4002972908691338No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGAAACTAACC281020.32860556971356286No Hit
TGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGG247690.2896317470726368No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA217230.25401390615926717No Hit
GTCTCAGAGAGGAGCCTTAGCCCTGGACTCCAAGGCCTTTCCACTTGGTG194750.22772733151276195No Hit
CAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGC183250.21428001797028823No Hit
GGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGAT180080.21057323675901501No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG178020.2081644136374936No Hit
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG161010.18827408291075637No Hit
GGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGACAGCCTGCGAA159610.18663701865341173No Hit
CTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTT158480.18531567393141216No Hit
CGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCAC157350.18399432920941255No Hit
CGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACC148160.17324817169155746No Hit
ACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCG143970.16834867223564745No Hit
TCGGGCCGAGATGTCTCGCTCCGTGGCCTTAGCTGTGCTCGCGCTACTCT143650.16797448611968296No Hit
CGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGG139940.16363626583771967No Hit
ATGCAGTCAGCAATGACTTAAATTAGTCAGGATAGCAGGCATCTGGGGTT137760.16108712292271163No Hit
CCTCTACCACCCCTGGGGGCCTCCTCCAAGGAGCAGGTGGTTAGATGTCC132600.15505337180278428No Hit
GCAGTGACGTTGGTGCTTATAACTATGTCTCCTGGTACCAACAACACCCA130400.15248084225552844No Hit
TGGACAGGCGATCATCATCACCTGCACTGGAACCAGCAGTGACGTTGGTG129270.15115949753352884No Hit
GCGGGATGAACCGAACGCCGGGTTAAGGCGCCCGATGCCGACGCTCATCA120910.1413838852539566No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA113700.13295300432863177No Hit
ACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTTGCAGGGACAGCAGA104450.1221366869140333No Hit
TCGGGAATTCACATACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTG97610.11413845868529239No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97430.11392797899506238No Hit
AGCACGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTACTGCTCTG93990.10990547824844416No Hit
AGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTG93700.10956637208085133No Hit
GCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAG93070.10882969316504623No Hit
AGACACTGGCGTGCCTGCTTCAAAGCCTCCCACCTATCCTACACAGGTAA92730.10843212041683399No Hit
CTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGC88680.10369632738665845No Hit
TAGTGATCAGTACCATATACAGTTTCTCCATAGGCTGGTAGACATATCTT88330.10328706132232228No Hit
TGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC86520.10117057110389815No Hit
CGCTCTCAGGACATCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCT86010.10057421198157974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)