FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004455696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9108386
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACG524510.57585394382715No Hit
AATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCATAATGATGGCG474840.5213217797313376No Hit
GCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATG273020.2997457507839479No Hit
AGACACTGGCGTGCCTGCTTCAAAGCCTCCCACCTATCCTACACAGGTAA261820.28744939004561293No Hit
TGCAAGTCGAGCGAGACCTTCGGGTCTAGCGGCGGACGGGTGAGTAACGC260030.2854841681061826No Hit
GAACAGGAAACCTTGGGCTTACGGCGTGCGGGCTTTTCACCCGCATTATC230260.25280000210794756No Hit
CTGAGGACAATGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAA213670.23458601776428886No Hit
CTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACA213230.23410294644956856No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT197890.21726132379545618No Hit
GAGAAGGTACGCTGCCGATGGTGAGTAGACATGCTCTACAAAGCTATTGG179230.1967747084939088No Hit
GTTAGGTTTAGTAGGCAAATCCGCTAGACTATTAATGAGATCTGATAGCA169910.18654237973665147No Hit
AATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCC151350.16616555337026778No Hit
TGTGGATAGGGGTGAAAGGCTAATCAAACTCGGTGATAGCTGGTTCTCCC148950.16353061892633886No Hit
AGAAGGTACGCTGCCGATGGTGAGTAGACATGCTCTACAAAGCTATTGGC147610.16205944719514523No Hit
TCATAAACAAGTATCGGAATATTAACCGATTTCCCATCGACTACGCCTTT134600.14777590673034718No Hit
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATGA120150.1319114055991918No Hit
GGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGAT118200.12977052136349954No Hit
AGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATG118090.12964975353481945No Hit
AAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGCTAATCAAACTCG114080.12524721723475488No Hit
GGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCT113490.12459946251728901No Hit
AAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCATAATGATGGC111850.12279892398060425No Hit
AGCAGGTTAATATTCCTGCACCGTCGTATGATGCGATGGGGGGACGGATC108510.11913197354613649No Hit
GTGGAATTTCTACTGCTTCCAGCCTCCACCTTGGAAAACGGGCAAGGGAG102270.11228114399192128No Hit
GGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGACAGCCTGCGAA100940.11082095115424401No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA99520.10926194827491939No Hit
TGAGGACAATGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAAG99160.10886670810833006No Hit
ATAAGGTACGCCCTAGTAGTTTGACCACTTTACTGTGGAAGGATGAACGG97200.10671484497912144No Hit
TAAGTTAAGAGGTTTTTATGGGTGGCGTACAATCTACTTTTTCTAACTCT94630.10389326934541422No Hit
TGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACAC93050.10215860416982767No Hit
CAGACACTGGCGTGCCTGCTTCAAAGCCTCCCACCTATCCTACACAGGTA92530.10158770170697641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)