FastQCFastQC Report
Tue 13 Sep 2022
EGAF00004455697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9108386
Sequences flagged as poor quality0
Sequence length50-120
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCATAATGATGGCG487780.5355284679415212No Hit
ACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACG411820.4521327927911707No Hit
AGACACTGGCGTGCCTGCTTCAAAGCCTCCCACCTATCCTACACAGGTAA238640.2620003148746661No Hit
GCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATG238590.2619454204070842No Hit
GAACAGGAAACCTTGGGCTTACGGCGTGCGGGCTTTTCACCCGCATTATC231070.2536892924827736No Hit
TGCAAGTCGAGCGAGACCTTCGGGTCTAGCGGCGGACGGGTGAGTAACGC197060.21635007563359743No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT195580.2147251993931746No Hit
TCATAAACAAGTATCGGAATATTAACCGATTTCCCATCGACTACGCCTTT154670.16981054601770282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG148920.16349768224578975No Hit
CTGAGGACAATGTATGGTGGGTAGTTTGACTGGGGCGGTCTCCTCCTAAA140230.1539570237800638No Hit
GAGAAGGTACGCTGCCGATGGTGAGTAGACATGCTCTACAAAGCTATTGG136350.14969721309571202No Hit
AATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCGTAACGATGGCC133860.14696346861013576No Hit
TGTGGATAGGGGTGAAAGGCTAATCAAACTCGGTGATAGCTGGTTCTCCC126480.13886104519505432No Hit
AAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCATAATGATGGC124290.13645666751496918No Hit
CTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACA122230.13419501545059687No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA121750.13366802856181106No Hit
GTTAGGTTTAGTAGGCAAATCCGCTAGACTATTAATGAGATCTGATAGCA116800.12823347627120765No Hit
GGCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGAT112580.1236003832072993No Hit
AGAAGGTACGCTGCCGATGGTGAGTAGACATGCTCTACAAAGCTATTGGC110510.1213277522494106No Hit
CTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATGA106010.11638725016704388No Hit
GGGTATTCCGTTCTCGGTGGTTCCGGGTATTACCGCAGCTTCTGGTTGCT102000.11198471386697928No Hit
CCTCCCGTAGGAGTCCGGACCGTGTCTCAGTTCCGGTGTGACTGATCATC95150.10446417180826548No Hit
TAATCTTGCGACCGTACTCCCCAGGCGGAATGCTTAATCCGTTAGGTGTG92000.10100582035060877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)