FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004455698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148308
Sequences flagged as poor quality0
Sequence length50-120
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA128261.1169477178596683No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA115841.0087885828540775No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA48590.42314431319820117No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC38840.33823677967931953No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC37330.32508699756511317No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC22640.19715964706333144No Hit
ACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAA22050.19202165272731705No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC22000.19158622947850226No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG16510.14377675675863966No Hit
GGGGCAAAAAAAAGAAGCAAGTTCTGAAGTTCACTCTTGATTGCACCCAC15500.13498120713258116No Hit
GAGTTGCTACCAATGCTTTGCCTGGTGTGGCTCCCTTCTTGCCAGGTGTG15190.13228158298992954No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG14440.12575023425770787No Hit
CGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCA13930.12130891711979713No Hit
TAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGT13580.11826095437809368No Hit
GAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGG12500.10885581220369449No Hit
TGCCGAAAAGAGAGGCAAACGCCAGGTGCTTATTAGGCCGTGCTCCAAAG12370.10772371175677606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)