FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004455699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148308
Sequences flagged as poor quality0
Sequence length50-120
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA115191.0031280806194853No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA112790.982227764676376No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC37510.3266545212608464No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC35440.3086279987599146No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA34190.29774241753954517No Hit
ACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTACCCAAATAAA21330.18575155794438428No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC21200.18461945749746583No Hit
CCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC20530.17878478596334782No Hit
CGTATGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTCATCA13460.11721593858093822No Hit
CTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCCAGTGAG13290.11573549953496796No Hit
TAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGT12170.10598201876151694No Hit
GAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGG11540.10049568582645074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)