FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004455700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504959
Sequences flagged as poor quality0
Sequence length50-120
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA89400.35689206889214553No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA88720.354177453603033No Hit
TGTGGATCGGAGTGAAAGGCTAATCAAGCTCGGAGATAGCTGGTTCTCCT76810.3066317652304888No Hit
TGTGGATAGGGGTGAAAGGCTAATCAAACTCGGTGATAGCTGGTTCTCCC74400.29701084927936944No Hit
AGGTGGCTGTGCTGGGGGCGCCCCACAACCCTGCTCCCCCGACGTCCACC63030.2516208848128852No Hit
GAGAAGGTACGCTGCCGATGGTGAGTAGTCACGCACTACAAAGCTATTGG57610.2299838041261354No Hit
ACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGGCACTGTCC56990.22750871371547396No Hit
GCAGATGACCGACGTCCACATCCTCGCCATCGACCTGGCGAAGCGCAGCT55410.22120122524959493No Hit
GGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGAA49130.19613095463837932No Hit
CGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGG48050.19181950682625942No Hit
TCAAAGGCAATTCCGAGGTTGAGCCCCGGGATTTCACCCCTGACTTAAAT46140.18419463152889928No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC44900.17924445070757644No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA39980.1596034106745859No Hit
AGAAGGTACGCTGCCGATGGTGAGTAGTCACGCACTACAAAGCTATTGGC39040.1558508542455186No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCC37210.1485453454527599No Hit
ACATTTTGGGAGGCAACCGCCCCAGTTAAACTACCCATCAGGCACTGTCC34930.13944340007161796No Hit
GGATAACGTTCGGAAACGGACGCTAATACCGCATACGTCCTACGGGAGAA33520.13381456542801698No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT33140.13229757453115998No Hit
TCATAAACAAGTATCGGAATATTAACCGATTTCCCATCGACTACGCCTTT28580.11409368376887605No Hit
AGGGAGCTTGACTGTGAGAGTGACAGCTCGAGCAGGGACGAAAGTCGGGA27680.1105008105921095No Hit
CAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAA26690.10654865009766627No Hit
TTGTGGATAGGGGTGAAAGGCTAATCAAACTCGGTGATAGCTGGTTCTCC26580.10610952115383925No Hit
AGCTACGGCGCGGGCCTGATGCTCGGCGCTCTTCACCGCCACATAATGCA25530.1019178357809449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)