FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004455701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2504959
Sequences flagged as poor quality0
Sequence length50-120
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA90340.36064462532121283No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA83850.3347360176354184No Hit
TGTGGATCGGAGTGAAAGGCTAATCAAGCTCGGAGATAGCTGGTTCTCCT60750.24251893943174319No Hit
TGTGGATAGGGGTGAAAGGCTAATCAAACTCGGTGATAGCTGGTTCTCCC58090.23190000315374423No Hit
AGGTGGCTGTGCTGGGGGCGCCCCACAACCCTGCTCCCCCGACGTCCACC56780.22667037664089512No Hit
ACTCTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGGCACTGTCC56680.22627116851014328No Hit
TCAAAGGCAATTCCGAGGTTGAGCCCCGGGATTTCACCCCTGACTTAAAT48560.19385546829309383No Hit
GAGAAGGTACGCTGCCGATGGTGAGTAGTCACGCACTACAAAGCTATTGG45850.18303692794971893No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC43070.17193894191481776No Hit
GCAGATGACCGACGTCCACATCCTCGCCATCGACCTGGCGAAGCGCAGCT37730.15062122773266948No Hit
GGCTCAGAGCGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGAA36330.14503231390214372No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA35580.1420382529215049No Hit
ACATTTTGGGAGGCAACCGCCCCAGTTAAACTACCCATCAGGCACTGTCC34730.13864498381011425No Hit
AGCTACGGCGCGGGCCTGATGCTCGGCGCTCTTCACCGCCACATAATGCA32950.1315390790827315No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCC32380.129263592737446No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT31330.12507190736455168No Hit
CGGCCGCAAGGTTAAAACTCAAATGAATTGACGGGGGCCCGCACAAGCGG31230.12467269923379984No Hit
TCATAAACAAGTATCGGAATATTAACCGATTTCCCATCGACTACGCCTTT29420.11744703206719152No Hit
AGAAGGTACGCTGCCGATGGTGAGTAGTCACGCACTACAAAGCTATTGGC29270.11684821987106375No Hit
CAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACAA29110.1162094868618608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)