FastQCFastQC Report
Sun 11 Sep 2022
EGAF00004455706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2645505
Sequences flagged as poor quality0
Sequence length50-120
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTAC413041.5612898104520687No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA283521.071704646182865No Hit
CCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACT232050.8771482193380848No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA207080.7827617033420841No Hit
GGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGC136080.5143819422000715No Hit
ACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTA121600.45964759091364404No Hit
CAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTC87120.32931330691115684No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA79690.3012279319071406No Hit
GAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCT79140.29914893375744894No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC65510.24762757961145415No Hit
GACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCT65190.2464179806879972No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC58580.22143220292533938No Hit
CCTCCTGTGCAAGAACATGAAGCACCTGTGGCTCTTCCTCCTGCTGGTGG49170.1858624345824332No Hit
CCTAGGGCTCAGAGCACTGCAGCAGATCATTTCATATTGCTTCCGTGGAG48460.1831786369710131No Hit
TAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAA46070.17414444501144394No Hit
ACCTCCTGTGCAAGAACATGAAGCACCTGTGGCTCTTCCTCCTGCTGGTG45280.17115824766915957No Hit
AATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAG42440.16042305722347908No Hit
CTCCTGTGCAAGAACATGAAGCACCTGTGGCTCTTCCTCCTGCTGGTGGC40160.15180466489384825No Hit
CATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCC38510.14556767044477328No Hit
AGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCT38020.14371547209322985No Hit
ATAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGA37800.14288387283335316No Hit
AGGAACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAG36840.1392550760629823No Hit
TACTCCTCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACC36650.13853687670217973No Hit
AACTGCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTT36590.13831007690403155No Hit
TAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGT35920.13577747915804356No Hit
ATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGT34950.13211088242131463No Hit
GGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCG34050.12870888544909195No Hit
GAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGG33880.12806628602100545No Hit
AGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTC32680.12353029005804186No Hit
TAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTT31130.1176712952725472No Hit
AGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGA28080.10614230553334808No Hit
GAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTG27950.10565090597069368No Hit
CAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAG27810.10512170644168127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)