FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004455710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27953035
Sequences flagged as poor quality0
Sequence length50-120
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGT2800401.0018232367254576No Hit
GGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCA2277390.8147201189423617No Hit
GGCATGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTA1983510.7095866334371206No Hit
GCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGA1217680.4356163829795226No Hit
CTCGGATGCGACGGCGCGGCTTTAAGGATTCAAGCTCGCGGCTCGGCGCT1078660.3858829640502364No Hit
AGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTC868720.3107784181574559No Hit
TAGACCGCCTGGGGAGTACGGTCGCAAGACTAAAACTCAAATGAATTGAC719080.2572457695559713No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA680670.24350486449861347No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA597630.21379789350244077No Hit
GAGGAGGAGGCTACCTAGATTAATACTTTAGGATAGTGGACGTTACTCGC583500.20874298622671922No Hit
ACATTTTGGGAGGCAACCGCCCCAGTTAAACTACCCACCAGGCACTGTCC556260.19899806944040244No Hit
TATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGC524770.18773274529939238No Hit
AATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGCATAATGATGGCG510870.1827601188922777No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG503980.18029527026313957No Hit
GGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAA486100.173898827086218No Hit
CGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGA465550.16654721034764206No Hit
GCTCAGATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGATG458580.164053742285945No Hit
CGGGTGAGTAATGCTTAGGAATCTGCCTATTAGTGGGGGACAACGTTCCG399790.14302203678419895No Hit
TGTGGATAGGGGTGAAAGGCTAATCAAACTCGGTGATAGCTGGTTCTCCC393820.14088631162948853No Hit
ACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACG391890.14019586781900428No Hit
CTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTAACA385640.1379599746503376No Hit
TGTCCACCGAGGCCTCCCAGAACATCACCTACCACTGCAAGAACAGCGTG362500.12968180378266617No Hit
TTGTAAAGCACTTTAAGCGAGGAGGAGGCTACCTAGATTAATACTTTAGG347260.12422980188018941No Hit
ACACGACGCTCCCCTACCCATCCACACACCTGCCAGAAAATCCTTGGATC345570.12362521636738193No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA338270.12101369314637928No Hit
TCGAAGGCAATTCTGTGGTTGAGCCACAGGCTTTCACCCCCGACTTAATC329070.11772245840210196No Hit
CGGAGCGGGCCTCCGAGTACGTGGCCGAGGCCATGTTCGCCGCGCGTTCC311040.11127235378913239No Hit
GGATGATCACGGCCACGATCATGAGATCCGCGCCGATCACGGACATGATC307940.1101633507774737No Hit
GCTAGTAGACATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTT293330.10493672690639853No Hit
GTGCTTCCAGTCATGTACATACATCGACAGAGTGCGTACCTACTGGGTCG289510.10357014900170948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)