FastQCFastQC Report
Mon 5 Sep 2022
EGAF00004455716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28344480
Sequences flagged as poor quality0
Sequence length50-120
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCT2573980.908106269721653No Hit
GGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCT2262090.7980707354659531No Hit
AAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTGGAGGTT1680710.5929584878607757No Hit
AACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCAT1213500.42812568796464073No Hit
CTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTT1100550.38827665915903203No Hit
TACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTC1037570.3660571652752141No Hit
GGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTG973230.3433578601547815No Hit
GCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGG821980.28999650020039175No Hit
AAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTGGAGGT737210.26008944245934307No Hit
GACACGGGGAGGTAGTGACAATATGTAACAATATACGGCTTTATCGTCGT721280.2544693005481138No Hit
CTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACC678250.23928821414257734No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA675690.23838504005012617No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG642380.22663319277686522No Hit
CATGTGTGCGAGTTTTTGGGTGGCAAACCCATGAGCGTAATGAAAGTGAA594460.20972690273379507No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAG573690.2023991973040253No Hit
ACGGACCAAGGAGTCTAACATGTGTGCGAGTTTTTGGGTGGCAAACCCAT542870.19152582795662507No Hit
GGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTC515170.18175320203439965No Hit
CAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGC512830.1809276444655185No Hit
AGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTC453430.1599711831016127No Hit
AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAACA443580.15649607965995496No Hit
CTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATG434360.15324324171761133No Hit
TCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTC417150.14717151276015647No Hit
ACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTT413380.14584144778807018No Hit
GAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTGGAGGTTCC403360.14230636794183557No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA393070.13867603145303778No Hit
CACATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTC389030.13725070983838827No Hit
CAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCA380710.134315394037922No Hit
GCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCG353530.12472622535322574No Hit
AGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTGGAGGTTC312970.1104165608259527No Hit
CAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCT312440.11022957556462493No Hit
TTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGG309220.10909355190146372No Hit
TCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGA308350.10878661383098226No Hit
CAATAACGCAGGTGTCCTAAGGGGGACTCATGGAGAACAGAAATCTCCAG300820.10613001191060835No Hit
ACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAG291860.1029689025870293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)