FastQCFastQC Report
Tue 27 Sep 2022
EGAF00004455717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28344480
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCT2131310.751931240227374No Hit
GGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCT2102480.7417599476159027No Hit
AAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTGGAGGTT1760070.6209568847267616No Hit
AACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCAT1053920.3718254841859861No Hit
CTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTT927890.3273618002517598No Hit
GGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTG858800.30298668382697447No Hit
TACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTC853740.30120150378486393No Hit
AAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTGGAGGT794250.28021329020677044No Hit
GCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGG772740.2726245110159015No Hit
GTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGCAACAA730260.2576374659192901No Hit
CTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACC688780.24300322320254245No Hit
CCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTG579580.20447720332142272No Hit
GACACGGGGAGGTAGTGACAATATGTAACAATATACGGCTTTATCGTCGT544510.19210442385960158No Hit
CATGTGTGCGAGTTTTTGGGTGGCAAACCCATGAGCGTAATGAAAGTGAA493560.17412914260554435No Hit
GGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTC486430.17161366163711594No Hit
CAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCGC432320.15252352486268933No Hit
GAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTGGAGGTTCC427820.15093591415330251No Hit
ACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAG421020.14853685797022914No Hit
CAACGTTATCGTCACAGCCCATGCATTTGTAATAATCTTCTTCATAGTAA397880.14037301090018234No Hit
ACGGACCAAGGAGTCTAACATGTGTGCGAGTTTTTGGGTGGCAAACCCAT390470.13775874526539206No Hit
AGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTC390040.13760704024205064No Hit
AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAACA376550.13284773613768888No Hit
CACATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTC360630.12723112225025826No Hit
ACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCAGACCCAGGTCTTCATTT339350.119723487606758No Hit
AGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTGGAGGTTC332220.11720800663832959No Hit
TCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTGCGCCTC322540.11379287960124865No Hit
TCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGA319100.1125792394145174No Hit
CAAGATGGTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCT318330.11230758158202234No Hit
CAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCA313330.11054356968270365No Hit
GTGTGCGGCCCCAACTCCAGCGCGAGGCAAAACCCTTTTTGCGTGAGATA311910.11004259030329715No Hit
CTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATG304650.10748124502548645No Hit
GCAGACCAGACTTCGCTCGTACTCGTGCGCCTCGCTTCGCTTTTCCTCCG301330.10630994112433884No Hit
TTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGG300800.10612295586301106No Hit
ACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAG290200.10238325063645551No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)