FastQCFastQC Report
Thu 5 Jan 2023
EGAF00004455723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004455723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25588437
Sequences flagged as poor quality0
Sequence length50-120
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAACCAGAAGTACAGGAATATTAACCTGTTTTCCATCGACTACGCTTTT1704370.6660703817118646No Hit
AGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAACA1159670.45320079534361557No Hit
CGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGACGTGGTAGCCTGCGA1046710.40905585597119515No Hit
GGATAACGTTCGGAAACGGACGCTAATACCGCATACGTCCTACGGGAGAA855660.33439322612787953No Hit
CTGGTTGATTCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATG701100.27399094364380283No Hit
GGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCA684780.2676130628846146No Hit
TCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAACA664810.2598087565879854No Hit
CATGTGTGCGAGTTTTTGGGTGGCAAACCCATGAGCGTAATGAAAGTGAA636810.24886631410898602No Hit
TGTGGATCGGAGTGAAAGGCTAATCAAGCTCGGAGATAGCTGGTTCTCCT599290.23420344118712683No Hit
CAAGTGAAGAAGCGCATACGGTGGATGCCTTGGCAGTCAGAGGCGATGAA567810.22190100942859461No Hit
ACATTTTAGGAGGCAACCGCCCCAGTTAAACTACCCATCAGGCACTGTCC524970.20515907243572554No Hit
ACATTTTGGGAGGCAACCGCCCCAGTTAAACTACCCACCAGGCACTGTCC399840.15625807860011146No Hit
GGATGCCTTGGCACCAAGAGCCGATGAAGGACGTTGTGACCTGCGATAAG391840.1531316664632545No Hit
AAAAGTTAGCGGATGACCTGTGGATCGGAGTGAAAGGCTAATCAAGCTCG388740.1519201817602224No Hit
ATCGCCTGCACGCCCGGAGTTAAGCCCCGGAATTTCACAGACGACGCGAC381750.14918847915564362No Hit
TCATTACGGCGATTCCAAAAACCAACAAAATAGAACCGCACGTCCTATTT340440.13304446848394844No Hit
ATCATTACGGCGATTCCAAAAACCAACAAAATAGAACCGCACGTCCTATT335420.1310826448680707No Hit
TCGAAAGCACGCTCAGGGTTAAGCCCCAAGATTACACTTCCGACGCGATC321520.1256505037802817No Hit
ACGGACCAAGGAGTCTAACATGTGTGCGAGTTTTTGGGTGGCAAACCCAT320560.12527533432385884No Hit
GGCTGGAATCTTAGGTAAATCCGGGATTTCAAGGCCGAGAGCTGATGACG311600.12177375273057905No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG278130.10869362595300369No Hit
AAGGGGGACTCATGGAGAACAGAAATCTCCAGTAGAACAAAAGGGTAAAA268410.10489503520672247No Hit
AATCATTACGGCGATTCCAAAAACCAACAAAATAGAACCGCACGTCCTAT264160.10323412875901722No Hit
TAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAAC262290.10250332992202689No Hit
GGGTCTATTCCCAGCGACTAGACGCCCTATTAAGACTCGCTTTCGCTACG261260.10210080435940655No Hit
GGGGTTAGCTAACGCGAAGCTCTTGATCGAAGCCCCGGTAAACGGCGGCC258040.10084242347432161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)